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mouse anti cd80 monoclonal primary antibody  (Proteintech)


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    Structured Review

    Proteintech mouse anti cd80 monoclonal primary antibody
    Mouse Anti Cd80 Monoclonal Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+cd80+monoclonal+primary+antibody/CD80%2FB7-1+Antibody/pm41764552-138-10-17
    Average 96 stars, based on 107 article reviews
    mouse anti cd80 monoclonal primary antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Extracellular vesicles from TNF-α and TGF-β1-treated ADSCs promote tissue-engineered cartilage regeneration by modulating macrophages via the miR-378a-3p/SIRPα axis.
    Article Snippet: To detect macrophage polarization markers, cells were fixed with 4% paraformaldehyde (PFA, Solarbio, China) for 15 min, permeabilized with 0.2% TritonX-100 (IT9100, Solarbio, China) for 10 min, and blocked with 5% BSA (SW3015, Solarbio, China) for 30 min at room temperature. .. The cells were then incubated overnight at 4°C with a mouse anti-CD80 monoclonal primary antibody (66406-1-Ig, 1:200, Proteintech, USA) and a rabbit anti-CD206 polyclonal primary antibody (ab64693, 1:200, Abcam, Cambridge, UK). .. Following primary antibody incubation, the cells incubated with goat polyclonal secondary antibodies against mouse IgG-H&L (Alexa Fluor® 488) (ab150113, 1:500, Abcam, Cambridge, UK) and rabbit IgG-H&L (Alexa Fluor ® 594) (ab150080, 1:500, Abcam, Cambridge, UK) for 1h at room temperature.



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